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1、Production of Artemisinin by Artemisia annua Hairy Root Culture in a Internal Loop Airlift BioreactorLIU Chun-Zhao VANG Yu-Chun OUYANG Fan( !3tate I r) Lnbrntir) of Biochemical Engineering Inst Jute of C/ieniirn/ 3feto/ur .77ie Cue Am&my of Srieiicev . Beijing 1(0)8()YE He-Chun LI Cuo- FengInstitute
2、 of Bian . 77ie Cae 4cIemj Srnw . Beijing I tO93 )Abstract A self-made internal lcop airlift bioreactor was used for production of artemisinin bv Artemisia an- nun L. hairy root multi-plate culture . The hairy rent cultures grew evenlv either between the two meshes or at- taching to the stainless st
3、eel meshes in the bioreactor . Under the optimal conditions of 12 liZd light cycle and25 , the dry weight and artemisinin yield reached 22. 57 QL and 374. 4 mgZ L respectively after 20 days . Meantime , the kinetics of sugar, phosphate , nitrate and ammonium uptake during the course of culture were
4、studied .Key words Artemisia annua Internal lcop airlift bioreac- tor, Hairy emit , Artemisinin , KineticsT iae (day)250Hg. 1 Schematic diagram of the internal lcop airlift bioreactor1. Bioreactor; 2. Stainless steel mesh ; 3 . .Yir inlet ; 4. .1ir outlet ; 5 . Air filter; 6. Flowmeter; 7. Air stora
5、ge tank ; 8 . 4O W fluores-cent lamp; 9. Peristaltic pump; 10. Sterile water inlet ; I I . Sterilewater Bask ; 12. Internal lcop. 10 J 5 20Fig. 3 Artemisiiu n synthesis in the bioreactorPhosphate4012040G-F ( Q ) . .1 nev vav for producing plant secondary metabolites hairy rant culture in tfiro by Ri
6、 plasmid transfor- mation. Bot Yes ( lII I -I= I I ) , 1994, 7. 29 - 41 ( in Chi- nesed2 Toivonen L, Ojala M . Kauppinen V . Indole alkaloid productionby hairy nxit cultures of Cmlmrentiius roseus -. kinetics and fermentation . Bioteclirml Lett , l9Rl .12 : 519 - 524Yuri Y( II ) , Ye H-CInduction of
7、 hairy rant from Ariemisia aruiua th Agrobacterium rliimgenes nod i u culture in ritro. .4cfn Bot Min ( ) , 1994, 36 : 165 - 170 I in Chinese)4 Weathers P J . Cheetham R D . Follansbee K . Tech K .Arlemisinin pnxluction by transformed roots of .4rfcziiisifi niiiizt-n. Biotechnol Lett , 1994, 16 : 12
8、81 - 12865 ChaRerjee C , Correll M J , Weather P J , Wyslouzil B E , &al- eerz D B . Simplified acoustic window mist bioreactor. Biotech- not 7er/i, 1997, 11: 155 - 1586 Dilorio A .1, Cheetham R D . Weathers P J . Carbon dioxide im- proves the growth of hairy rants cultured on solid medium and in nu
9、trient mists . .Appl Microbiol Biotechnol, 1992 , 37 : 457 -Fi .5 KinetiCS Of ammonium and nitrate uptake in culture7 l/hitney P J . Ixovel bioreactor for the growth of rants trans- formed by Agrobocterium rliizogenes . Enxyme Micro Technol , 1992, 14. 13 - 178 then X-K( ) . 1an K-D( HQ ) , Luo Z-Y(
10、 / ) .Determination of artemisinin content by ultraviolet meter. Chin Fluum Anal - fi fl c.1 , 1983, 3 ( 1 ) : 24 = 26 ( in Chi- nese)9 Dubois M , Lilles . .1, Hamilton J K, Rexers P .4, Smith F. Colorimetrie methods for the determination of sugar and related substances. .4nn/ Chem, 1956, 28 : 350 -
11、 35710 Chen P S. Toribara T , Huber Warner . Microdetermination ofphosphorus . .4no/ Chem . 1956,28 : 1756 = 17591 I Ieatherbum M Y . Phenol- h,vpxHorite reaction for determina- tion of ammonia . Anal Chem. 1967.39: 971 - 97412 I/ang J-L( T ) , Zh Z-Y( KG I ) . Analytical Chem-istry . Vol . 3 . Beijing : Chemistry Industry Publications, 1988.687 - 688 ( in Chinese)
