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1、1Adrenomedullin and endometriosisAbstract Objective To investigate the adrenomedullin (ADM and endometrium, endometriosis (the relationship between the Ems and immunohistochemical SP method detected 33 cases of the Ems patients with ectopic, eutopic endometrium Organization (Research group ADM expre
2、ssion in normal endometrial tissue of 26 cases of non-endometriosis patients as controls. Results the Ems patients and ectopic endometrium and eutopic endometrium of ADM expression was significantly higher than in normal endometrium, the difference was significant (H = 137.10, P 0.01. Conclusion ADM
3、 is an autocrine growth factor in human endometrial cells, and participate in the proliferation of endometrial blood vessels, blocked ADMs secretory pathway, it may become a new target for treatment of Ems points. Keywords: endometriosis, adrenomedullin, immune histochemistry ABSTRACT ObjectiveTo st
4、udy the relationship between adrenomedullin (ADM) and 2endometriosis.MethodsThe ADM in eutopic and ectopic endometrium in 33 patients with endometriosis (study group) and in normal endometrium in 26 non endometriosis patients (control group) was detected by immunohistochemical method. ResultsAdrenom
5、edullin expression in eutopic and ectopic endometrium of the study group was higher than that of the control group, the difference was statistically significant (H = 137.10, P 0.01). ConclusionADM may be an autocrine growth factor of human endometrial cells participating in endomembrane vascular hyp
6、erplasia, blocking the ADM autocrine pathway may become a new target in treating endometriosis. KEY WORDS Endometriosis; Adrenomedullin; Immunohistochemistry Endometriosis disease (Ems is a common disease of women of childbearing age, its pathogenesis is still not clear in recent years, along with t
7、he deepening of understanding of the the Ems pathogenesis of research and found that the Ems patients eutopic endometrium of a variety of molecular biological level a biological peptide, originally isolated 3from human pheochromocytoma conducive to blood reflux after planting and growth abnormalitie
8、s. adrenomedullin (ADM on human uterine distribution of its specific binding sites in vitro and in vivo both ADMs immune response to material existence, ADM can induce cAMP production of endometrial cells in the human endometrium. These results indicated that of ADM may be an autocrine growth factor
9、 for human endometrial cells, and participate in endometrial vascular hyperplasia. In this study, immunohistochemical SP method detected 33 cases of the Ems patients with ectopic expression of ADM in the eutopic endometrial tissue, and normal endometrial tissue of 26 cases of non-Ems patients as con
10、trols, in order to understand the ADM Ems the relationship between the results reported below. 1 Materials and Methods 1.1 General Information 1.1.1 The study group Select in January 2006 to January 2007, 33 cases of hospital in-patients, age 25 to 45 years are due to the Ems hysterectomy, ovarian e
11、ndometrial cyst excision or (and) the endometriotic lesion resection other internal and surgical 4diseases. hysterectomy immediately after scraping the endometrium of 33, set at -20 . endometriotic lesion tissue samples of 33 were pathologically confirmed. 1.1.2 The control group January 2006 to Jan
12、uary 2007, outpatient or inpatient 26 cases, age 25 to 45 years, no other internal and surgical diseases of which 10 cases of menstrual disorders by diagnostic curettage in 16 cases of uterine fibroids hysterectomy surgery, postoperative instantly take the endometrium, set at -20 , the pathological
13、examination revealed 12 cases of proliferative endometrium, secretory endometrium (n = 14). 1.2 Methods 1.2.1 The specimen processing All specimens with 40 g / L formaldehyde solution fixed, paraffin embedded, sectioned to 4 m thick. 1.2.2 Immunohistochemical staining Handled as follows: dewaxing, w
14、ashing, high pressure repair of each slice using the SP method of immunohistochemistry staining to observe the expression of ADMs kit was purchased from Wuhan Boster. 51.2.3 the results to determine Select three horizons, counting the percentage of positive cells in the field of vision of 400 times
15、the mean value, number of positive cells from 10% to 25% (+ 26% 50% (, 51% to 75% ( greater than 75% (. of ADM low expression for the ADM number of positive cells 75%. 2 results The average expression of the control group, the proliferative phase endometrial tissue ADM 32%, 30% of the average expres
16、sion of the secretory phase endometrial tissue ADM in the study group the average expression of the reign of the endometrial tissue of ADM for 92% ectopic the average expression of the membrane organization of ADM was 95%. research eutopic and ectopic endometrium and the control group of ADM expression, a statistically significant difference Share the free paper download center ht
