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1、1Acute myocardial infarction bone marrow and peripheral blood cardiac transcription factors Nkx2-5Author: Xu Zhiwei Xu Xia Zhang Zhongming Red endothelial Keywords myocardial infarction; monocytes; Nkx2-5 Abstract: Objective To observe the mice myocardial infarction, bone marrow and peripheral blood
2、 mononuclear cells of cardiac-specific transcription factor Nkx2-5 change method with a large dose of isoproterenol (10mg.kg continuous injection three days caused myocardial acute shortage of mice model of hemorrhagic infarction, 30 min after injection of the three days, by enucleation method perip
3、heral blood, separating the posterior limbs of the femur, tibia, a bone marrow fluid flushing the marrow cavity, bone marrow and peripheral blood mononuclear cells isolated by density gradient centrifugation, the normal group with myocardial ischemia and infarction. cells Western blotting analysis,
4、relatively normal group and myocardial infarction, bone marrow and peripheral blood 2mononuclear cells Center changes of muscle-specific transcription factor Nkx2-5 results in normal bone marrow mononuclear cells Nkx2-5 expression of normal peripheral blood mononuclear cells Nkx2-5 expression; myoca
5、rdial infarction, bone marrow and peripheral blood Nkx2-5 expression was significantly increased compared with the normal group significant (P 0.05) conclusion in normal bone marrow without culture or induce i.e. presence monocytes Nkx2-5 expression increased after acute myocardial infarction, perip
6、heral blood mobilization. Keywords: myocardial infarction; monocytes; Nkx2-5 Abstract: Objective To examine the cardiac specific transcription factor Nkx2-5in the bone marrow and peripheral blood after myocardial infarction.Methods The mouse model of myocardial infarction was formed by sequential in
7、jection of large dose Iso (10mg.kgfor3days.Bone marrow and peripheral blood mononuclear cells (PBMCwere then collected from the model and the normal control mice to compare the expression of Nkx2-5in bone marrow and PBMC through 3Western blot-ting.Results Nkx2-5was found to be positive in normal bon
8、e marrow, but weak or negative in normal PBMC.The Nkx2-5expression became positive and obviously increased in both bone marrow and PBMC after myocardial infarction (P 0.05.Conclusion The mononuclear cells, which express Nkx2-5, reside in bone marrow and are mobilized into peripheral blood after myoc
9、ardial infarction, participating in repairing the infarction zone. Keywords: myocardial infarction; mononuclear cells; Nkx2-5 Transcription factor Nkx2-5 the Nkx2-of NK-type homeobox gene family members cardiac progenitor (precursor cells (cardiac pro-genitor cell, one of the early signs of CPC diff
10、erentiation, with significant heart-specific expression characteristics 1 The CPC also be that some parts of existence cardiac 2-3, but in recent years has been confirmed at the nucleotide level, the existence of the CPC in the bone marrow mononuclear cell populations without training or induction o
11、f expression Nkx2 the -5 by chemotaxis in vitro experiments showed that the group of cells to the 4infarcted myocardium chemokines 4 In this study, the isoproterenol-induced mouse acute myocardial ischemia and infarction model based on further confirmed at the protein level in the bone marrow Nkx2-5
12、 expression after myocardial infarction, peripheral blood and bone marrow Nkx2-5 expression were significantly up-regulated in monocytes. 1 Materials and methods 1.1 Animals Kunming mice, of either gender, body weight (20 2g, provided by the Experimental Animal Center of Xuzhou Medical College. 1.2
13、Reagent goat anti-Nkx2-5 polyclonal antibodies were purchased from Santa Cruz, USA, alkaline phosphatase-labeled rabbit anti-goat IgG were purchased from Beijing Zhongshan Golden Bridge Biotechnology company, NBT.BCIP purchased from Sigma (USA), isoproterenol hydrochloride were purchased from Shando
14、ng Kefeng Pharmaceutical Co., Ltd., the mouse monocyte separation was purchased from on Haiqi Kang Biotechnology Co., Ltd., the improved Western purchased from cell lysates Haixi Tang biotech companies. 51.3 mouse model of myocardial infarction produced eight mice were randomly divided into normal g
15、roup, and myocardial infarction group (n = 4). Myocardial infarction mice each daily intraperitoneal injection of isoproterenol use chronic unpredictable mild stress as three days of continuous injection normal mice given saline instead of isoproterenol eight mice were recorded one day before the in
16、jection of normal ECG, ECG recording again three days after injection finished 30min observe whether caused by myocardial ischemia and infarction. 1.4 Western blotting Nkx2-5 protein expression was measured 1.4.1 Peripheral blood mononuclear cells obtained have recorded ECG, the normal group and myocardial infarction randomly from each of the two mice obtained by enucleated peripheral blood, each mouse was about 0.8 1.0ml peripheral blood can be obtai
