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1、1About the significance of the diagnosis of fungal keratitis the KOH smear microscopy methodAuthor: YANG Li-ping, Huang Wenming, Fan Yi, Li Rui Zhuang Keywords KOH smears Abstract Objective: To investigate KOH smear microscopy method for diagnosis of fungal keratitis significance about Zhanjiang the
2、 etiologic fungal keratitis. Methods: 102 cases of fungal keratitis patients the corneal ulcers specimens for KOH coated slides examined, fungal culture and strain identification.: the KOH smear microscopy method and fungal culture-positive rates were 97.1% (99/102, 94.9% (93/98, the the fungus dete
3、ction rate differences There was no significant sex (P 0.05.93 were culture positive specimens, were isolated from 18 genera and 33 species of fungi, including Fusarium, Curvularia genus Aspergillus accounted for 36.6%, 27.9%, and 7.5%, respectively. conclusions: The KOH smear microscopy Law is the
4、early diagnosis of the disease is simple, effective way to Fusarium and Curvularia Zhanjiang main 2pathogens of fungal keratitis. Keywords keratitis, fungal, culture Abstract Objective: To investigate the diagnostic value of potassium hydroxide based smear assay for mycotic keratitis and the pathoge
5、nic spectrum in Zhanjiang area. Methods: The corneal scrapings from 102 patients with fungal keratitis were subjected to 10% KOH based smear before microscopic observation, fungal culture and strain identification. Results: The positive rates of fungal infection of fungal keratitis detected by KOH b
6、ased smear and fungal culture were 97.1% (99/102) and 94.9% (93/98), respectively, and there was no significant difference in the fungal detection efficiency between the two methods (P 0.05). The pathogenic fungi belonged to 18 genus and 33 species, of which, Fusarium, Curvularia and Aspergillus acc
7、ounted for 36.6%, 27.9% and 7.5%, respectively. Conclusion: The KOH based smear is simple and effective in the early diagnosis of fungal keratitis. Fusarium and Curvularia are the predominant pathogens of mycotic keratitis in Zhanjiang district. 3Keywords: keratitis; fungus; culture Showed an increa
8、sing trend in the prevalence of fungal keratitis in our country, its pathogens so far have been as many as 70 species 1 the fungal keratitis improper disposal will result in perforation of the cornea, the loss of that even enucleation and treatment success and recovery of vision depends on early, ra
9、pid and correct diagnosis. although KOH the smear microscopy law is still developing countries, rapid diagnosis of fungal keratitis detection methods, but the domestic reports about its significance to the diagnosis of the disease rare 26. analysis in our hospital from October 2003 to December 2005,
10、 the diagnosis and treatment of 102 cases of fungal keratitis mycological examination results, aimed at understanding the diagnosis of the KOH smear microscopy of fungal keratitis significance. 1 Materials and Methods 1.1 The clinical data 102 patients, 71 male and 31 females, aged 6 to 85 years (me
11、an 48.7 years, disease duration from 3 to 60 days (mean 13.9d, are monocular illness, including 55 cases of the right eye, left eye 47 cases, 89 cases of farmers, 4workers, students four cases of .58 patients had a clear history of ocular trauma caused by trauma, foreign body grain or grain dust (20
12、 cases, suspicious dust (20 cases, soil (5 cases, wood chips (four cases sugarcane leaves (3 cases, welding crumbs (two cases, cement powder (1 cases), bamboo (1, orange leaves (1 stabbed grass (1 cases. 1.2 Specimen Collection 1% tetracaine line suffering from ocular surface anesthesia for 2 min in
13、 aseptic corneal ulcers scraped with a scalpel, placed in sterile plastic tubes containing a small amount of sterile saline censorship. 1.3 KOH smear microscopy With sterile platinum ear picked the right amount the submission of corneal ulcers organization placed in the slide, smear try to spread a
14、thin layer of corneal tissue, add 1 drop of 10% KOH solution coverslip, look under the microscope fungi . 1.4 fungal cultures Remaining corneal ulcer tissue was inoculated on two Sabouraud medium (SDA 5tubes bevel, 27 cultured for 2 to 3 weeks, to observe whether the fungal growth. 1.5 Identificatio
15、n of bacteria Two the SDA test tubes in both fungal growth and colony morphology initially identified pathogens. Whichever colonies transplanted glucose potato agar (PDA) or Czapek medium plate, 27 cultured for 2 weeks observe the shape and speed of growth, colonies transplanted in the PDA or Czapek
16、 medium line, microplate microscope every day the growth of fungal mycelium and conidia. based on colony color, morphology, growth rate, and the presence of liquid exudation, as well as colonies smears and trace training microscope mycelium color, shape and conidial morphology, structure and sporulation way Features, strain identification 78. 1.6 statistical Using paired chi-square test. 2 Results 102 corneal ulcer specimens were d
