[精选]Strukturbiochemie中子散射

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1、,PD Dr. Regine Willumeit GKSS Forschungszentrum Geesthacht Max-Planck-Str. 1 21502 Geesthacht Tel.: 04152 871291 Mobil: 0170 431 6 430 Email: willumeitgkss.de,Strukturbiochemie I,Donnerstag, 17:00 19:00 Uhr 10.05.2007 24.05.2007 07.06.2007 21.06.2007 05.07.2007 - Exkursion zur GKSS,Termine,Neutronen

2、- und Rntgenstreuung (gelste Systeme) - 3 h Fluoreszenzspektroskopie (verschiedene Varianten) - 2 h Surface Plasmon Resonance -1 h Infrarot- und CD-Spektroskopie - 2 h Massenspektroskopie - 1 h Zeta Potential - 1 h,Themen der Vorlesung:,Allgemeine Vorbemerkungen:,Strukturbiochemie - was ist das?,Erf

3、orscht die Struktur von Proteinen, Nukleinsuren, Lipiden, Zucker. Komplexen (Ribosomen, Membranen, Zellen),Erforscht die Funktion und Dynamik von Moleklen Untersucht die Wechselwirkung von Moleklen,(Protein)strukturanalyse,Die wichtigsten Datenbanken,EM,NMR,http:/ndbserver.rutgers.edu/NDB/NDBATLAS/,

4、URL funktioniert gerade mal nicht,Literatur,Google / Wikipedia !,Grundlagen: Wechselwirkung mit Materie Kontrastvariation Aufbau eines Instrumentes Datenaufnahme und Auswertung Strukturanalyse an Beispielen,Neutronen- und Rntgenstreuung (gelste Systeme),Accessable Size Regimes,Introduction to Scatte

5、ring,electromagnetic waves,electromagnetic waves short wavelength,particles,charged particles,Only Elastic Scattering!,Inelastic scattering,Transfer of energy/momentum into/from the sample atoms,Interaction of Radiation with Matter,Interaction of Radiation with Matter,Interaction with electrons,Inte

6、raction with electrons,Scattering strength is proportional to Z,Interaction with electron spin possible,Interaction with nuclei (protons and neutrons),Scattering strength does not vary systematically,Interaction with nuclear spin possible,Interaction with electrons and electron spin possible,Atomic

7、Scattering factors / length,H,R.Winter, F. Noll: Methoden der biophysikalischen Chemie, Teubner (1998),X-Rays,Neutrons,atomic mass / g mol-1,Comparison Neutron- and X-ray-scattering length some relevant elements 10-12 cm,nX-ray 1H-0.370.28 2H0.670.28 12C0.661.68 14N0.941.96 16O0.582.24 31P0.514.2 32

8、S0.284.48 56Fe0.956.72,Neutron Scattering Length of biological relevant elements 10-12 cm,F. Sears (1986), H. Glttli und M. Goldmann (1987),X-Ray Speciality: Anomalous Scattering (ASAXS),Neutrons X-Rays Intensitylowhigh H-sensitivity highnone Isotope-sensitivitystrongnone Heavy elements low high Spi

9、n-sensitivity strong average Penetration depth high low Sample size/amount large small Measurement time long short Interaction with nuclei electron shell electron shell unsystematica Z Radiation damagenone high,To Remember:,To Remember:, H. Zabel, Bochum,Contrast Variation,When the monster came, Lol

10、a, like the peppered moth and the arctic hare, remained motionless and undetected. Harold of course, was immediately devoured.,The Concept of Contrast Variation,Contrast = Difference of Scattering Length Densities,Contrast = Difference of Scattering Length Densities,p(R) = rParticle(R) - rLM(R),p(R)

11、 = rParticle(R) - rLM,r(R) = Scattering Length Densitiy = Sum of Scattering Length of all Atoms in a Volume,The Concept of Contrast Variation,Contrast = Difference of Scattering Length Densities,Change of solvent composition Change of sample composition - Isotopic substitution Change of scattering p

12、rocess,Usually: Combination of them,Examples: Polymer-Surfactant Interaction,Juha Merta and Per Stenius: Helsinki University of Technology, Espoo, Finland Vasil M. Haramus,Materials:,Synthetic Cationic Polymer: Poly(diallyldimethylammonium chloride) PDADMAC M = 5 50104 , 30 104,Anionic Surfactant: S

13、odium hexadecanoate, NaPal - C15H31COONa, CAC 0.1 mM, CMC = 1.6 mM, Kraft point 62 oC,Application:,How are the two components organized?,Internal Contrast Variation,C15H31COONa - NaPal(H) C15D31COONa - NaPal(D),Solvent D2O,Where is the surfactant NaPal and where the polymer PDADMAC ?,Examples: Polym

14、er-Surfactant Interaction,Surfactant,H,D,PDADMAC/NaPal/D2O,Examples: Polymer-Surfactant Interaction,Internal Contrast Variation,Examples: Polymer-Surfactant Interaction,Conclusion: Shell Structure of PDADMAC/NaPal: Surfactant Core & Polymere Shell,J. Merta, V.M. Garamus, R. Willumeit, P. Stenius (20

15、02) Langmuir, 18, 7272-7278,Distance Distribution,Composition of bacterial ribosomes,R.Jnemann und K.H.Nierhaus: Naturwissenschaftliche Rundschau 10 (1993) 391-400,D.I. Svergun et al.: JMB 271 (1997) 602-618,Ribosome Structures,D.I. Svergun et al.: JMB 271 (1997) 602-618,Ribosome Structures,D.I. Sve

16、rgun et al.: JMB 271 (1997) 602-618,Shape and RNA-distribution,Modelling,Ribosome Structures,Neutron Scattering Length of biological relevant elements 10-12 cm,F. Sears (1986), H. Glttli und M. Goldmann (1987),Spin Contrast Variation,deuterated,isotopic substitution,In situ Structures of 14 Proteins,Willumeit et al.: BBA 1520 (2001) 7-20,ca. 100 ,Yusupov et al.: Science 292 (2001) 883-896,Comparison of L4 position,Willumeit et al.: BBA 1520 (2001) 7-20,ca. 100 ,Crystallography,50S subunit E.coli

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