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1、常用缓冲溶液的配制方法1 .甘氨酸-盐酸缓冲液(0.05mol/L )X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI ,再加水稀释至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07, 0.2 mol/L甘氨酸溶液含15.01克/升2 .邻苯二甲酸-盐酸缓冲液(0.05 mol/L )X毫升0.2 mol/L邻苯二甲酸氢钾 + 0.2 mol/L HCl ,再加水稀释到20毫升pH(20 C)XYpH(20 C)XY2.22.42.62.8
2、3.0555554.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263邻苯二甲酸氢钾分子量=204.23 , 0.2 mol/L邻苯二甲酸氢溶液含 40.85克/升3.磷酸氢二钠-柠檬酸缓冲液pH0.2mol/LNa2HPO4（毫升）0.1mol/L柠檬酸（毫升）2.20.4010.602.41.2418.762.62.1817.822.83.1716.833.04.1115.893.24.9415.063.45.7014.303.66.4413.563.87.1012.904.07.7112.294.28.2811.724.
3、48.8211.184.69.3510.654.89.8610.145.010.309.70pH0.2mol/LNa2HPO4（毫升）0.1mol/L柠檬酸（毫升）5.210.729.285.411.158.855.611.608.405.812.097.916.012.637.376.213.226.786.413.856.156.614.555.456.815.454.557.016.473.537.217.392.617.418.171.837.618.731.277.819.150.858.019.450.55Na2HPO4分子量=14.98 , 0.2 mol/L 溶液为 28.40
4、 克/升。Na2HPO4 2H2O 分子量 =178.05 , 0.2 mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量=210.14 , 0.1 mol/L 溶液为 21.01 克/升。4.柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7 H2O氢氧化钠（克）NaOH97%盐酸（毫升）HCl （浓）最终体积（升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.382661561
5、2610 使用时可以每升中加入 1克克酚，若最后 pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5.柠檬酸-柠檬酸钠缓冲液（0.1 mol/L ）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48
6、.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸CeHsO? H2O:分子量210.14 , 0.1 mol/L溶液为21.01克/升。柠檬酸钠 Na3 C6H5O7 2H2O:分子量 294.12 , 0.1 mol/L 溶液为 29.41 克/ 毫升6 .乙酸-乙酸钠缓冲液(0.2 mol/L )pH（18 C）0.2 mol/LNaAc（毫升）0.3 mol/LHAc（毫升）2.60.759.253.81.208.804.01.808.204.22.657.354.43.706.30pH（18 C）0.2 mol/LNaAc（毫升）0.3 mol
7、/LHAc（毫升）4.85.904.105.07.003.005.27.902.105.48.601.405.69.100.904.64.905.105.89.400.60Na2Ac 3H2O 分子量=136.09 , 0.2 mol/L 溶液为 27.22 克/升。7.磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2)pH0.2 mol/LNa2HPO4（毫升）0.3 mol/LNaH2PO4（毫升）5.88.092.05.910.090.06.012.387.76.115.085.06.218.581.56.322.577.56.426.573.56.531.568.56.637.5
8、62.56.743.556.5pH0.2 mol/LNa2HPO4（毫升）0.3 mol/LNaH2PO4（毫升）7.061.039.07.167.033.07.272.028.07.377.023.07.481.019.07.584.016.07.687.013.07.789.510.57.891.58.57.993.07.06.86.949.555.051.045.08.094.75.3Na2HPO4 2H2O 分子量 =178.05 , 0.2 mol/L 溶液为 85.61 克/升。Na2HPO4 12H2O 分子量 =358.22 , 0.2 mol/L 溶液为 71.64 克/升。
9、Na2HPO4 2H2O 分子量 =156.03 , 0.2 mol/L 溶液为 31.21 克/升。(2)磷酸氢二钠-磷酸二氢钾缓冲液(1/15 mol/L)pHmol/L/15Na 2HPO4（毫升）mol/L/15KH 2PO4（毫升）pHmol/L/15Na 2HPO4（毫升）mol/L/15KH 2PO4（毫升）4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509.008.007.006.005.004.007.177.387.738.048.348.677.008.009.009.509.
10、759.9010.003.002.001.000.500.250.1008.18Na2HPO4 2H2O 分子量 =178.05 , 1/15M 溶液为 11.876 克/升。KH2PO4分子量=136.09 , 1/15M 溶液为 9.078 克/升。8.磷酸二氢钾-氢氧化钠缓冲液（0.05M ）X毫升0.2M K2PO4 + Y毫升0.2N NaOH加水稀释至29毫升pH(20 C)X （毫升）Y （毫升）pH(20 C)X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806
11、.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液（18C）pH0.04M巴比0.2V盐酸pH0.04M巴比0.2N盐酸妥钠溶液妥钠溶液（毫升）（毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M 溶液为8.25克/升10 . Tris -盐酸缓冲液（0.05M , 25）50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X
