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1、关于 Diaclone,成立于1986年,坐落在贝桑松(贝桑松),法国 25年的经验和专业知识,Diaclone仍然致力于提供卓越的单克隆抗体和免疫分析的发展。,细胞凋亡细胞因子细胞因子受体趋化因子CD标记粘附分子,研究领域覆盖:,1,产品,Diaclone质量管理系统认证ISO9001:2008确保持续维护高质量的产品和服务。,质量,2,单克隆抗体,Diaclone mAbs非常具体,范围广泛的高质量的鼠单克隆抗体蛋白质包括人类CD抗原、细胞因子、细胞因子/趋化因子受体,粘附分子和细胞凋亡相关抗原。,宿主: 老鼠 特异性: 人类 版式: -非结合的 -异硫氰酸荧光素 -偶联物 - 藻红蛋白
2、- 维生素H - 多甲藻黄素-叶绿素蛋白复合物 应用:流式细胞术、免疫沉淀反应、免疫印迹、免疫组织化学、 生物分析,3,举例1：,4,举例2：,5,举例3：,6,举例4：,7,举例5：,8,Biologically Active Antibodies,Diaclone offers a broad range of biologically active, Azide Free antibodies suitable for induction, activation, neutralization and inhibition studies.,9,ELISA,Diaclone ELISA
3、(Enzyme Linked Immuno-Sorbent Assay) are highly sensitive assays designed for the in-vitro quantitative and qualitative determination of cytokines, CD antigens, apoptosis markers and other analytes from human serum, plasma and cell culture supernatant.,Formats Available:,ELISA Kits: Pre-Coated Plate
4、s, Biotinylated Detection Antibody, standard(s), Control(s) (where available), Buffers, Streptavidin-HRP and TMB, Stop reagent and plate sealers. ELISA Sets: Capture Antibody, Biotinylated Detection Antibody, standard(s), Streptavidin-HRP and TMB. HS Kits: High Sensitivity (HS) kits for the detectio
5、n of low expressing proteins with sensitivities below 1.5pg/ml.,10,Diaclones ELISA products can be used in three species: Human, Murine, Rat （小鼠）,Characteristics: Highly sensitive assay Fast procedure Accuracy and reliability are guaranteed as all our reagents have been validated according ISO 9001:
6、 2000 quality systems Recognizes both Natural and Recombinant antigen Specificity No cross reactivity with other human cytokines tested Standard Calibration to NIBSC（英国国家生物制品检定所）,11,ELISpot,酶联免疫斑点技术,在研究免疫应答机制时,以往人们常用（ELISA）检测体液中游离的细胞因子(CK)或抗体,但由于游离的循环抗体或CK半衰期不同,使之在体液中不断地被代谢或与靶器官结合,而不能确切地反应体内抗体及CK水平。
7、而酶联免疫斑点(ELISPOT)技术则可以在单细胞水平上进行细胞测控。因其具有较高的特异性和敏感性（ELISA敏感度的400倍）,目前正被国内外广泛应用。 原理：将抗原或特异性单抗预先包被在贴有PVDF膜或硝酸纤维素膜的96孔微孔板上,将经适量抗原刺激后的免疫细胞加入微孔中,于 37CO2培养箱中孵育,捕获抗原或抗体与免疫细胞分泌出的抗体或细胞因子结合,将细胞和未结合的成分洗掉后,加入酶（或荧光素）标记的单抗或多抗,与被检测的抗体或细胞因子结合。加入底物后,可在有相应抗体、细胞因子的位置产生有色斑点,每个斑点代表一个分泌抗体或细胞因子的细胞。,12,Formats Available ELIS
8、pot Kits: Pre-coated PVDF plate(s), Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer. ELISpot Sets: Capture and Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer. ELISpot Pair: Capture and Detection An
9、tibodies. Dual ELISpot Sets: Capture and Detection antibodies for two analytes, Alkaline phosphatase conjugate, blocking reagent, Ready-to-use BCIP/NBT substrate buffer, Peroxidase conjugate, BSA, Ready-to-use AEC substrate buffer. Dual Fluorospot Sets: Capture antibody for two analytes, FITC-conjug
10、ated Detection antibody for cytokine 1, anti-FITC antibody green fluorescence conjugate, biotinylated detection antibody for cytokine 2, streptavidin-phycoerythrin conjugate, BSA.,13,DIAplex,Diaclone DIAplex is a sensitive multiplex fluorescent bead-based immunoassay for the simultaneous quantificat
11、ion of multiple analytes from a single sample by flow cytometry,Test multiple sample types including serum, plasma and cell culture supernatants Compatible with most commercially available flow cytometers Small sample volume required Takes less time than a conventional ELISA and provides data that would normally require multiple ELISA in a smaller sample volume,14,
