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1、超临界CO2萃取小麦胚芽油工艺及其品质的研究Optimization and Evaluation of Wheat Germ Oil Extracted by Supercritical CO2,牛丽亚 Niu LiYa 2010.10.22,Outline,Backgrounds Objectives Results and discussion Conclusions Future research,What is wheat germ?,Wheat germ oil,Wheat germ oil has high content of bioactive compounds: toco
2、pherol (highest content of all vegetable oils) polyunsaturated fatty acids, especially linoleic acid (55%),Wheat germ oil has been demonstrated to reduce plasma and liver cholesterol in animals and to delay aging.,Wheat germ oil is used in products such as foods, biological insect control agents, ph
3、armaceuticals and cosmetic formulations.,Defatted wheat germ, 30% protein,Rich in amino acids, especially the essential amino acids ( lysine, methionine, and threonine).,Existed problems,Low oil yield,Solvent residue,Loss of bioactive components,Defatted meal denaturation,Solution,Non-explosive, non
4、-toxic, non-solvent residues.,Dont need the traditional refining processes.,Avoid fatty acids oxidation and protein in defatted wheat germ denaturation.,Supercritical CO2 extraction,Objectives,The effects of pretreatments (water content, particle size) of wheat germ on oil yield were investigated. E
5、xtraction parameters (pressure,temperature and extraction time) were optimized using response surface method.,The physical properties and chemical composition of oils and defatted wheat germ obtained by supercritical CO2 and solvent were compared.,Results and Discussion Effect of pretreatment of whe
6、at germ on oil yield, Optimization of extraction parameters using RSM,Uncoded and coded levels of independent variables used in the RSM design.,Experimental scheme and results obtained from RSM.,Analysis of variance for the response surface model.,3-D plot of response surface for the effect of varia
7、bles on oil yield.,A maximal oil yield of 10.46% was achieved under optimal conditions: wheat germ particle size 60-80 mesh; water content 4.37%; pressure 30MPa; temperature 40; extraction time 1.7h; CO2 flow rate 25L/h.,Fatty acid composition of wheat germ oils extracted with different methods.,Phy
8、sical and chemical characteristics of wheat germ oils extracted with different methods.,Antioxidant activity of wheat germ oil assessed by DPPH radical scavenging assay,Comparative amino acid profile of defatted wheat germ with different methods (g/100g),Conclusions,A second-order polynomial model w
9、as sufficient to describe and predict the response variable of the oil yield for supercritical CO2 extraction of wheat germ oil. A maximal oil yield of 10.46% was achieved under optimal conditions of wheat germ particle size 60-80 mesh; water content 4.37%; pressure 30MPa; temperature 40; extraction
10、 time 1.7h; CO2 flow rate 25L/h. Compared with the solvent extraction using petroleum ether, wheat germ oil and defatted meal obtained from supercritical CO2, contained more important bioactive components.,Future research,Protein in the defatted meal will be developed. VE and polyunsaturated fatty acids in the oil will be enrichment. Microcapsulation technology will be used to change the oil form.,Thank you for your attention.,
