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1、J Toxicol Pathol 2010; 23: 99101 Case Report The First Case of Feline Infectious Peritonitis-like Pyogranuloma in a Ferret Infected by Coronavirus in Japan Yoshiko Michimae1, Shin-ichi Mikami2, Kazuo Okimoto3, Kaoru Toyosawa1, Izumi Matsumoto1, Mami Kouchi1, Takatoshi Koujitani1, Tadashi Inoue1, and
2、 Takaki Seki1 1Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd., 3198 Kasugade-naka, Konohana-ku, Osaka 554-0022, Japan 2Animal Health Products, Dainippon Sumitomo Pharma Co., Ltd., 1551 Ebie, Fukushima-ku, Osaka 553-0001, Japan 3Research Administration, Dainippon Sumitomo Pharma Co
3、., Ltd., 3394 Enoki-cho, Suita, Osaka 564-0053, Japan Abstract: A male ferret, which was purchased from abroad at 9 months of age, had shown significant weight loss starting at 13 months of age. The ferret subsequently showed decreasing motor activity and recumbency and was euthanized at 14 months o
4、f age. At necropsy, a white, quail egg-sized mass was found in the mesentery. Histopathologically, multifocal granulomas consisting of necrotic foci, macrophages, fibroblasts and plentiful fibrous connective tissues were observed in the mesenteric mass. Surrounding the granulomas, inflammatory cell
5、infiltration consisting of neutrophils, lymphocytes and plasmacytes was observed diffusely and significantly. Immunohistochemistry revealed small numbers of macrophages around necrotic foci that were positively stained for anti- mouse feline coronavirus. Electron microscopically, the cytoplasm of th
6、e macrophages contained viral particles, which were identified as coronavirus. The histopathological features in this ferret were similar to those in cats with feline infectious peritonitis (FIP). This was the first case in ferrets in Japan. (J Toxicol Pathol 2010; 23: 99101) Key words: granuloma, f
7、erret, feline infectious peritonitis, coronavirus, mesentery Feline infectious peritonitis (FIP) is a common disease in cats and some non-domestic felids that is caused by feline coronavirus (FCoV)1. This virus is included in the Coronaviridae family, a group of enveloped positive- stranded RNA viru
8、ses1. Although FIP manifests itself in effusive or noneffusive forms, the basic lesion is the same, a pyogranulomatous inflammation and vasculitis1. Testing by immunofluorescence assay, ELISA, electron microscopy, RT-PCR and sequence analysis may be useful in supporting a diagnosis of FIP1,2. Since
9、2006, some ferrets with clinical signs and visceral lesions similar to those in cats with FIP have been reported25. All cases in these reports were found in either Europe or the United States, and no case had been reported in Japan. Recently, we encountered a case of pyogranulomatous inflammation in
10、 the mesentery of a male ferret that was similar to the cases previously reported. This paper describes the histopathological features of our ferret, which is the first reported case of feline infectious peritonitis-like pyogranuloma in ferrets in Japan. A male ferret (Mustela putorius furo), which
11、was purchased from abroad at 9 months of age, had shown significant weight loss since 13 months of age. The animal subsequently showed decreasing activity and recumbency, and so it was euthanized by exsanguination via the carotid aorta under ketamine anesthesia. The procedures for animal care and ho
12、using were in compliance with the institutional guidelines for the care and use of laboratory animals. At necropsy, a white, quail egg-sized mass in the mesentery, enlarged spleen and white foci in the lungs were found. After necropsy, tissue samples from the mesenteric mass, liver, kidneys, heart,
13、gastrointestine, pancreas and urinary bladder were fixed in neutral buffered 10% formalin for histological examination. The tissue samples were then dehydrated, embedded in paraffin wax, sectioned at 3 microns and stained with hematoxylin and eosin (HE). The sections of the mesenteric mass, lung and
14、 spleen were also stained by the Gram, Ziehl-Neelsen and periodic acid-Schiff (PAS) methods. For immunohistochemical examination, the sections of the mesenteric mass were subjected to a labeled polymer method using Histofine Simple Stain Rat MAX-PO (MULTI) (Nichirei Biosciences Inc., Tokyo, Japan) f
15、or anti- mouse FCoV (MCA2194, 1:100, AbD Serotec, Oxford, UK) Received: 14 October 2009, Accepted: 8 January 2010 Mailing address: Yoshiko Michimae, Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd., 3198 Kasugade-naka, Konohana-ku, Osaka 554-0022, Japan TEL: 81-6-6466-5366 FAX: 81-6
16、-6466-5446 E-mail address: yoshiko-michimaeds-pharma.co.jp 100FIP-like Pyogranuloma in a Ferret in Japan Figs. 1 and 2. The mesenteric mass shows multifocal granulomas consisting of necrotic foci, macrophages, fibroblasts and plentiful fibrous connective tissues. HE stain. Fig. 3. Aggregated plasma cells are scattered around blood vessels surrounding granulomas in the mesenteric mass. HE stain. Fig. 4. Immunohistochemistry for FCoV. Small numbers of macrophages around necrotic foci show posit
