《2012 Recent Transmission of a Novel Alphacoronavirus, Bat Coronavirus HKU10, from Leschenault_s Rousettes to Pomona Leaf》由会员分享，可在线阅读，更多相关《2012 Recent Transmission of a Novel Alphacoronavirus, Bat Coronavirus HKU10, from Leschenault_s Rousettes to Pomona Leaf（13页珍藏版）》请在金锄头文库上搜索。
1、Recent Transmission of a Novel Alphacoronavirus, Bat Coronavirus HKU10, from Leschenaults Rousettes to Pomona Leaf-Nosed Bats: First Evidence of Interspecies Transmission of Coronavirus between Bats of Different Suborders Susanna K. P. Lau,a,b,c,dKenneth S. M. Li,dAlan K. L. Tsang,dChung-Tong Shek,e
2、Ming Wang,fGarnet K. Y. Choi,dRongtong Guo,f Beatrice H. L. Wong,dRosana W. S. Poon,dCarol S. F. Lam,dSylvia Y. H. Wang,dRachel Y. Y. Fan,dKwok-Hung Chan,dBo-Jian Zheng,d Patrick C. Y. Woo,a,b,c,dand Kwok-Yung Yuena,b,c,d State Key Laboratory of Emerging Infectious Diseases,aResearch Centre of Infec
3、tion and Immunology,bCarol Yu Centre for Infection,cand Department of Microbiology,d The University of Hong Kong, Hong Kong; Agriculture, Fisheries and Conservation Department, The Government of Hong Kong Special Administrative Region, Hong Konge; and Guangzhou Center for Disease Control and Prevent
4、ion, Guangzhou, Chinaf Althoughcoronavirusesareknowntoinfectvariousanimalsbyadaptingtonewhosts,interspeciestransmissioneventsarestill poorlyunderstood.Duringasurveillancestudyfrom2005to2010,anovelalphacoronavirus,BatCoVHKU10,wasdetectedin two very different bat species, Ro-BatCoV HKU10 in Leschenaul
5、ts rousettes (Rousettus leschenaulti) (fruit bats in the suborder Megachiroptera) in Guangdong and Hi-BatCoV HKU10 in Pomona leaf-nosed bats (Hipposideros pomona) (insectivorous bats inthesuborderMicrochiroptera)inHongKong.Althoughinfectedbatsappearedtobehealthy,Pomonaleaf-nosedbatscarry- ingHi-BatC
6、oVHKU10hadlowerbodyweightsthanuninfectedbats.Toinvestigatepossibleinterspeciestransmissionbetween thetwobatspecies,thecompletegenomesoftwoRo-BatCoVHKU10andsixHi-BatCoVHKU10strainsweresequenced.Ge- nomeandphylogeneticanalysesshowedthatRo-BatCoVHKU10andHi-BatCoVHKU10representedanovelalphacoronavirus s
7、pecies,sharinghighlysimilargenomesexceptinthegenesencodingspikeproteins,whichhadonly60.5%aminoacididentities. EvolutionofthespikeproteinwasalsorapidinHi-BatCoVHKU10strainsfrom2005to2006butstabilizedthereafter.Molecu- lar-clockanalysisdatedthemostrecentcommonancestorofallBatCoVHKU10strainsto1959(high
8、estposteriordensityre- gionsat95%HPDs,1886to2002)andthatofHi-BatCoVHKU10to1986(HPDs,1956to2004).Thedatasuggestedrecentin- terspeciestransmissionfromLeschenaultsrousettestoPomonaleaf-nosedbatsinsouthernChina.Notably,therapidadaptive geneticchangeinBatCoVHKU10spikeproteinby ?40%aminoaciddivergenceafte
9、rrecentinterspeciestransmissionwaseven greaterthanthe?20%aminoaciddivergencebetweenspikeproteinsofsevereacuterespiratorysyndrome-related Rhinolophus batcoronavirus(SARSr-CoV)inbatsandcivets.Thisstudyprovidedthefi rstevidenceforinterspeciestransmissionofcoronavi- rusbetweenbatsofdifferentsuborders. C
10、 oronaviruses (CoVs) infect a wide variety of animals, causing respiratory, enteric, hepatic, and neurological diseases of varying severity. Traditionally, CoVs have been classifi ed into groups 1, 2, and 3, based on genotypic and serological character- istics(29,79).Recently,thenomenclatureandtaxon
11、omyofCoVs wererevisedbytheCoronavirusStudyGroupoftheInternational CommitteeforTaxonomyofViruses(ICTV).CoVsarenowclas- sifi ed into three genera, Alphacoronavirus, Betacoronavirus, and Gammacoronavirus, which replace the three traditional groups (5).NovelCoVs,whichrepresentedanovelgenus,Deltacoronavi
12、- rus, have also been identifi ed (72, 73). While CoVs from all four genera can be found in mammals, bat CoVs are likely the gene source of Alphacoronavirus and Betacoronavirus, and avian CoVs arethegenesourceofGammacoronavirusandDeltacoronavirus(9, 41,73).CoVsareknowntopossesshighfrequencyofrecombi
13、na- tion and mutation rates, which may allow them to adapt to new hosts and ecological niches (21, 29, 35, 68, 71, 78). The severe acute respiratory syndrome (SARS) epidemic, causedbySARSCoV(SARS-CoV)(17,27,43),hasboostedinter- est in the discovery of novel CoVs in both humans and animals (12,20,36,
14、41,63,65,66,72).Inparticular,apreviouslyunknown varietyofCoVshavebeenidentifi edinbatsfromChinaandother countries,includingSARS-relatedRhinolophusbatCoVs(SARSr- Rh-BatCoVs) in horseshoe bats, suggesting that bats are impor- tant reservoirs of CoVs (8, 13, 30, 31, 33, 40, 49, 59, 67, 70). How- ever,
15、our understanding of the diversity, evolution, and interspecies transmission of CoVs in animals is still limited. For example,itremainsunknownifbatsarethedirectoriginofSARS- CoV in civets and humans, as the spike (S) protein of SARSr-Rh- BatCoV possesses only ?80% amino acid identity to that of cive
16、t SARSr-CoV, with signifi cant differences in the receptor binding domain (30, 32, 40, 51). Duringacontinuoussurveillancestudy,inanattempttobetter understandtheroleofbatsintheevolutionofCoVsandsearchfor other bat species which may have served as intermediate hosts for interspecies transmission of SARSr-CoVs, a potentially novel al- Received 24 May 2012 Accepted 16 August 2012 Published ahead of print 29 August 2012 Address correspondence to Patrick C. Y. Woo, pcywoohkucc.hku.hk, or Kwok-Yung Yue
