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1、荧光素名称 激发波长 (nm) 发射波长 (nm) 备注 1,5 IAEDANS 336 490 1,8-ANS 372 480 4-Methylumbelliferone 385 502 5-carboxy-2,7-dichlorofluorescein 504 529 5-Carboxyfluorescein (5-FAM) 492 518 5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH 5-Carboxytetramethylrhodamine (5-TAMRA) 542 568 5-FAM (5-Carb
2、oxyfluorescein) 492 518 5-HAT (Hydroxy Tryptamine) 370-415 520-540 5-Hydroxy Tryptamine (HAT) 370-415 520-540 5-ROX (carboxy-X-rhodamine) 578 567 604 591 5-TAMRA (5-Carboxytetramethylrhodamine) 548 542 552 568 6-Carboxyrhodamine 6G 518 543 6-CR 6G 518 543 6-JOE 520 548 7-Amino-4-methylcoumarin 351 4
3、30 7-Aminoactinomycin D (7-AAD) 546 647 7-Hydroxy-4-methylcoumarin 360 449, 455 9-Amino-6-chloro-2-methoxyacridine 412, 430 471, 474 ABQ 344 445 Acid Fuchsin 540 630 ACMA (9-Amino-6-chloro-2- methoxyacridine) 412, 430 471, 474 Acridine Orange + DNA 502 526 Acridine Orange + RNA 460 650 Acridine Oran
4、ge, both DNA & RNA 440-480 520-650 Acridine Red 455-600 560-680 Acridine Yellow 470 550 Acriflavin 436 520 Acriflavin Feulgen SITSA 355-425 460 Aequorin (Photoprotein) 466 Photoprotein AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP Alexa Fluor 350 346 342 442 441 Alexa F
5、luor 430 431 540 Alexa Fluor 488 495, 492 519, 520 Alexa Fluor 532 531, 532 553, 554 Alexa Fluor 546 556, 557 572, 573 Alexa Fluor 568 577, 578 603 Alexa Fluor 594 590, 594 617, 618 Alexa Fluor 633 632 650 Alexa Fluor 647 647 666 Alexa Fluor 660 668 698 Alexa Fluor 680 679 702 Alizarin Complexon 530
6、-560, 580 580, 624- 645 Alizarin Red 530-560 580 Allophycocyanin (APC) 630, 645 655, 660 AMC, AMCA-S 345 445 AMCA (Aminomethylcoumarin) 345 347 425 444 AMCA-X 353 442 Aminoactinomycin D 555 655 Aminocoumarin 346 350 442 445 Aminomethylcoumarin (AMCA) 345 347 425 444 Anilin Blue 600 Anthrocyl stearat
7、e 360-381 446 APC (Allophycocyanin) 630, 645 655, 660 APC-Cy7 625-650 755 APTRA-BTC = Ratio Dye, Zn2+ 466/380 520/530 Ratio Dye, Zn2+ APTS 424 505 Astrazon Brilliant Red 4G 500 585 Astrazon Orange R 470 540 Astrazon Red 6B 520 595 Astrazon Yellow 7 GLL 450 480 Atabrine 436 490 ATTO-TAG CBQCA 465 560
8、 ATTO-TAG FQ 486 591 Auramine 460 550 Aurophosphine G 450 580 Aurophosphine 450-490 515 BAO 9 (Bisaminophenyloxadiazole) 365 395 BCECF (high pH) 492, 503 520,528 BCECF (low pH) 482 520 Berberine Sulphate 430 550 Beta Lactamase 409 447, 520 BFP blue shifted GFP (Y66H) 381, 382, 445, 447, blue shifted
9、 GFP Blue Fluorescent Protein 383 448 (Y66H) Blue Fluorescent Protein BFP/GFP FRET Bimane 398 490 Bisbenzamide 360 461 Bisbenzimide (Hoechst) 360 461 bis-BTC = Ratio Dye, Zn2+ 455/405 529/505 Ratio Dye, Zn2+ Blancophor FFG 390 470 Blancophor SV 370 435 BOBO -1 462 481 BOBO -3 570 602 Bodipy 492/515
10、490 515 Bodipy 493/503 533 549 Bodipy 500/510 509 515 Bodipy 505/515 502 510 Bodipy 530/550 528 547 Bodipy 542/563 543 563 Bodipy 558/568 558 569 Bodipy 564/570 564 570 Bodipy 576/589 579 590 Bodipy 581/591 584 592 Bodipy 630/650-X 625 642 Bodipy 650/665-X 647 665 Bodipy 665/676 605 676 Bodipy Fl 50
11、4, 505 511, 513 Bodipy FL ATP 505 514 Bodipy Fl-Ceramide 505 511 Bodipy R6G SE 528 547 Bodipy TMR 542 574 Bodipy TMR-X conjugate 544 573 Bodipy TMR-X, SE 544 570 Bodipy TR 589 617 Bodipy TR ATP 591 620 Bodipy TR-X SE 588 616 BO-PRO -1 462 481 BO-PRO -3 544 570 Brilliant Sulphoflavin FF 430 520 BTC -
12、 Ratio Dye Ca2+ 464/401 533/529 Ratio Dye Ca2+ BTC-5N - atio Dye, Zn2+ 459/417 517/532 Ratio Dye, Zn2+ Calcein 494 517 Calcein Blue 373 440 Calcium Crimson 588, 589 611, 615 Calcium Green 501, 506 531 Calcium Green-1 Ca2+ Dye 506 531 Ca2+ Dye Calcium Green-2 Ca2+ 506/503 536 Ca2+ Calcium Green-5N Ca
13、2+ 506 532 Ca2+ Calcium Green-C18 Ca2+ 509 530 Ca2+ Calcium Orange 549 575 576 Calcofluor White 385, 395, 405 437, 440, 445 Carboxy-X-rhodamine (5-ROX) 576 601 Cascade Blue 377 398 399 420 423 Cascade Yellow 399 400 550 552 Catecholamine 410 470 CCF2 (GeneBlazer) CFDA 494 520 CFP - Cyan Fluorescent
14、Protein 430, 433, 436, (453) 474, 475, 476, (501) Cyan Fluorescent Protein CFP/YFP FRET Chlorophyll 480 650 Chromomycin A 436-460 470 Chromomycin A 445 575 CL-NERF (Ratio Dye, pH) 504/514 540 Ratio Dye, pH CMFDA 494 520 Coelenterazine Ca2+ Dye, bioluminescence (429) 465 Ca2+ Dye, bioluminescence, na
15、tive molecule Coelenterazine cp (Ca2+ Dye,) (430) 442 Ca2+ Dye, bioluminescence Coelenterazine f (437) 473 Ca2+ Dye, bioluminescence Coelenterazine fcp 452 Ca2+ Dye, bioluminescence Coelenterazine h (437) 464 Ca2+ Dye, bioluminescence Coelenterazine hcp (433) 444 Ca2+ Dye, bioluminescence Coelenterazine ip 441 Ca2+ Dye, bioluminescence Coelenterazine n (431) 467 Ca2+ Dye, bioluminescence Coelenterazine O 460 575 Coumarin Phalloidin 387 470 C-phycocyanine CPM Methylcoumarin 384 469 Methylcoumarin CTC 400-450 602 CTC Formazan Cy2 489 506 Cy3.1 8 554 568 Cy3.5 581 598 Cy3 514 552 55
