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1、第二节 基因工程及其应用 第六章从杂交育种到基因工程 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 能发光的水母 能否能否让热能否能否让热 带鱼也能发光带鱼也能发光? ? 设想设想 不
2、能发光的热带斑马鱼 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Evaluation only.Evaluation only. Created with Aspose.Slides
3、 for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .
4、NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 能产生人胰岛素的大肠杆菌 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspo
5、se Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 基因“嫁接 ” Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. T T T T A G C
6、C G G A A Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 1.什么叫基因工程? 2.基因工程 的基本操作步骤有哪些? 阅读教材 P102，解决以下问题： Evaluation
7、only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. v 又称基因拼接技术,或重组DNA 技术 。 v通俗的说，就是按照人们的意愿，把 一种生物的某种基因提取出来，加以 修饰改造，然后放到另一种生物的细 胞里，定向
8、的改造生物的遗传性状。 基因工程(gene engineering) Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 原 理： 操作水平： 结 果： 基因重组 DNA分子水平 定向地改造
9、生物的遗传性状， 获得人类所需要的品种。 基因工程(gene engineering) 优 点： 克服远缘杂交不亲和的障碍。 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 1 2345
10、 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 培育转基因大肠杆菌的关键步骤：培育转基因大肠杆菌的关键步骤： 1.ONE1.ONE 胰岛素基胰岛素基 因从人体因从人体 细胞内提细胞内提
11、 取出来取出来 2.TWO2.TWO 胰岛素基胰岛素基 因与运载因与运载 体体DNADNA连连 接接 3.THREE3.THREE 胰岛素基因胰岛素基因 导入受体导入受体( ( 大肠杆菌大肠杆菌) ) 细胞细胞 基因的“剪刀” 基因的“针线”基因的运载体 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 20
12、04-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 如:EcoRI 积极思考 1. 1.基因的基因的剪刀剪刀限制酶限制酶 基因操作的工具基因操作的工具 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty
13、Ltd.Copyright 2004-2011 Aspose Pty Ltd. CTTCATG AATTCCCTAA GAAGTACTTAA GGGATT GGCATCTTAA AATTCCGTAG 练习使用EcoRI 剪切目的基因 CTTCATG AATTCCCTAA GAAGTACTTAA GGGATT GGCATCTTAA AATTCCGTAG 目的基因 黏性末端 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspo
14、se.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 2. 2.分子针线分子针线DNADNA连接酶连接酶 基因操作基因操作的工具的工具 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Clien
15、t Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 使用DNA连接酶制作重组DNA分子 甲片段 CTTCATG AATTCCCTAA GAAGTACTTAA GGGATT 乙片段 GGCATCTTAA AATTCCGTAG 重组DNA分子 GGCATCTTAA AATTCCGTAG Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.
16、0.Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.Copyright 2004-2011 Aspose Pty Ltd. 3. 3.基因的运输工具基因的运输工具运载体运载体 常用的运载体有两类：常用的运载体有两类： 1 1）细菌细胞质的）细菌细胞质的质粒质粒 2 2）噬菌体噬菌体或某些或某些动植物病毒动植物病毒 基因操作基因操作的工具的工具 Evaluation only.Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0.Created with Aspose.Slides for .NET 3.5 Client
