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1、ProteomicsBio-Rad蛋白质组双向电泳实验操作手册ProteomeWorksTM System双向电泳实验流程l 样品制备（Sample preparation）l 固相预制胶条的水化（IPG strip rehydration）l 第一向等电聚焦（IEF）l 胶条的平衡（IPG strip equilibration）l 第二向SDS-PAGE电泳（SDS-PAGE electrophresis）l 凝胶的染色及检测（Detection/Staining）l PDQuest软件分析（Software analysis）l 质谱鉴定（Protein identification）目
2、录第一章 实验材料11 IPG预制胶条及载体两性电解质12 蛋白质定量试剂盒及其试剂13 试剂盒及其试剂14 化学试剂15 蛋白质Marker16 染色试剂17 注意事项第二章SDS-PAGE聚丙烯酰胺凝胶电泳2. 1 溶液的配制2. 2 SDS-PAGE凝胶的配制2. 3 操作方法2. 4 注意事项第三章双向电泳3. 1 溶液配制3. 2 操作步骤3. 3 注意事项附录1双向电泳完整的操作步骤附录2聚丙烯酰胺凝胶电泳凝胶的配置附录3细胞样品的一般处理步骤附录4组织样品的一般处理步骤第一章 实验材料11 IPG预制胶条及载体两性电解质（一）IPG预制胶条（美国Bio-Rad公司），-20冰箱保
3、存ReadyStrip IPG Strips, pH 310, 7 cm, 163-2000ReadyStrip IPG Strips, pH 47, 7 cm, 163-2001ReadyStrip IPG Strips, pH 310 nonlinear, 7 cm, 163-2002ReadyStrip IPG Strips, pH 36, 7 cm, 163-2003ReadyStrip IPG Strips, pH 58, 7 cm, 163-2004ReadyStrip IPG Strips, pH 710, 7 cm, 163-2005ReadyStrip IPG Strips,
4、 pH 310, 17 cm, 163-2007ReadyStrip IPG Strips, pH 47, 17 cm, 163-2008ReadyStrip IPG Strips, pH 310 nonlinear, 17 cm, 163-2009ReadyStrip IPG Strips, pH 36, 17 cm, 163-2010ReadyStrip IPG Strips, pH 58, 17 cm, 163-2011ReadyStrip IPG Strips, pH 710, 17 cm, 163-2012ReadyStrip IPG Strips, pH 310, 11 cm, 1
5、63-2014ReadyStrip IPG Strips, pH 47, 11 cm, 163-2015ReadyStrip IPG Strips, pH 310 nonlinear, 11 cm, 163-2016ReadyStrip IPG Strips, pH 36, 11 cm, 163-2017ReadyStrip IPG Strips, pH 58, 11 cm, 163-2018ReadyStrip IPG Strips, pH 710, 11 cm, 163-2019ReadyStrip IPG Strips, pH 3.95.1, 17 cm, 163-2020ReadySt
6、rip IPG Strips, pH 4.75.9, 17 cm, 163-2021ReadyStrip IPG Strips, pH 5.56.7, 17 cm, 163-2022 ReadyStrip IPG Strips, pH 6.38.3, 17 cm 163-2023ReadyStrip IPG Strips, pH 3.95.1, 11 cm, 163-2024ReadyStrip IPG Strips, pH 4.75.9, 11 cm, 163-2025ReadyStrip IPG Strips, pH 5.56.7, 11 cm, 163-2026ReadyStrip IP
7、G Strips, pH 6.38.3, 11 cm, 163-2027ReadyStrip IPG Strips, pH 3.95.1, 7 cm, 163-2028ReadyStrip IPG Strips, pH 4.75.9, 7 cm, 163-2029ReadyStrip IPG Strips, pH 5.56.7, 7 cm, 163-2030ReadyStrip IPG Strips, pH 6.38.3, 7 cm, 163-2031ReadyStrip IPG Strips, pH 310, 18 cm, 163-2032ReadyStrip IPG Strips, pH
8、310 nonlinear, 18 cm, 163-2033ReadyStrip IPG Strips, pH 47, 18 cm, 163-2034ReadyStrip IPG Strips, pH 36, 18 cm, 163-2035ReadyStrip IPG Strips, pH 58, 18 cm, 163-2036ReadyStrip IPG Strips, pH 710, 18 cm 163-2037ReadyStrip IPG Strips, pH 3.95.1, 18 cm, 163-2038ReadyStrip IPG Strips, pH 4.75.9, 18 cm,
9、163-2039ReadyStrip IPG Strips, pH 5.56.7, 18 cm, 163-2040ReadyStrip IPG Strips, pH 6.38.3, 18 cm, 163-2041ReadyStrip IPG Strips, pH 310, 24 cm, 163-2042ReadyStrip IPG Strips, pH 310 nonlinear, 24 cm, 163-2043ReadyStrip IPG Strips, pH 47, 24 cm, 163-2044ReadyStrip IPG Strips, pH 36, 24 cm, 163-2045ReadyStrip IPG Strips, pH 58, 24 cm, 163-2046ReadyStrip IPG Strips, pH 710, 24 cm, 163-2047（二）载体两性电解质（美国Bio-Rad公司），4冰箱保存Bio-Lyte 3/10 Ampholyte，40%，10ml 163-1112
