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1、李杰 吉红 刘品 西北农林科技大学动物科技学院,动物营养学分会饲料营养学术研讨会 2010.10.16-17 杨凌,二零一零年十月,研究背景,草鱼： 大型淡水养殖对象 产量占淡水鱼产量的20.2%(2008) 草鱼脂质蓄积过度,Highly Unsaturated Fatty Acids (HUFAs),PUFA Carbon chain lengths C20 Three or more double bonds.,HUFA的生理功能,HUFA in Atlantic salmon,(Kjr et al., 2008; stbye, T.-K.，2009； Todorevi et al.,
2、2008, 2009, 2010).,Marine fish,Freshwater fish,草鱼HUFA研究进展,Takeuchi （1991） 发现HUFA较LNA有着更佳的促进生长效应,曹俊明（1996，1997），吉红(2009)发现HUFA可降低草鱼肝脂蓄积及提高脂质代谢效率。,当前尚未对草鱼HUFA营养进行系统研究。,草鱼是否需要HUFA? 饲料HUFA是否有助于解决草鱼的过度蓄积问题？ 如果有作用的话，HUFA对于草鱼的作用机理是什么？,问题,健康草鱼,为期75天饲养试验,生长,代谢,生长速度,饲料效率,脂肪酸组成,抗氧化状态,代谢酶活性,Protocal,生物学性状,代谢相关基
3、因表达,Animals: 200 草鱼稚鱼(BW= 5.660.21 g Dietary treatments: 5 treatments,Feeding experiment,HUFA level: 0%, 0.26%, 0.56%, 0.83%, 1.13%,日常管理,饱食投喂 1日三次。 水温 28.01.3C 溶解氧 7.800.54 mg L1 pH值 7.50.29 氨氮 0.100.02 mg L1 光照 12h,FA composition of the hepatopancreas,Growth performance,Specific growth rate,Weight
4、gain,Feed utilization,Feed efficiency,Protein efficiency,y = 212X2 + 223.18X + 397.3; R2 = 0.9082;,Biological parameters,IPF index,Spleen ratio,- IPF development suppressed by n-3 HUFAs (Ma et al，2009；Ji et al，2009） - DHA inhibits T cell activation induced by LPS-DCs (Weatherill et al., 2005). - HUF
5、As incorporated into the membrane of immune cell might be oxidized causing a loss of cellular activity, thus impairing immune function.,Lipid content,Liver lipid content decreased with increasing dietary n-3 HUFAs content (Ibeas et al., 1996） Muscle lipid level level unchanged with increasing dietar
6、y fish oil (Richard et al., 2006).,Activities of lipid metabolism-related enzymes in the hepatopancreas,Liver LPL activity and gene expression increased with fish oil (Murphy, 1993; Zampelas, 1994; Richard , 2006). G6PDH was unchanged by taking fish oil (Menoyo et al,2004, Richard, 2006) MDH unchang
7、ed with fish oil (Zhou et al.,2008),Antioxidant status,Conclusion,Adequate dietary n-3 HUFAs promotes growth performance and lipid metabolism Excess dietary n-3 HUFAs exerts adverse effects, due to oxidative stress Dietary n-3 HUFAs inhibits lipid accumulation in fresh water fish,Supported by,National Natural Science Foundation of China (30771667) Foundation for Youth Key Teachers of Northwest A & F University.,Thanks for your attention,
