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1、4种黄连生物碱的抑菌作用(Antibacterial activity of 4 alkaloids from Coptis chinensis)Antibacterial activity of 4 alkaloids from Coptis chinensisObjective berberine, Huang Lianjian, and palmatine are 4 main kinds of Coptis chinensisIn order to study the difference of antibacterial properties of 4 alkaloids from
2、Coptis chinensis, the inhibition of them was studied in this experimentThe spectrum and antibacterial activity were studied. Methods 4 alkaloids extracted from the rhizome of Coptis chinensis were used as the experimentThe 19 common microorganisms were tested under the concentration of 2000 M g/raL
3、solution by the method of bacteriostatic circleThe drug sensitivity test was carried out, and the minimal inhibitory concentration (MIC) of sensitive bacteria was determined by turbidimetryTesting。 Results the sensitive range of the 4 alkaloids was the same, but the antibacterial activity was differ
4、ent, among which berberine was the most sensitiveThe antibacterial activity was the highest, followed by Huang Lianjian and Bama, and the alkaloid was the least; the 4 alkaloids of Coptis chinensis were gram positiveThe inhibitory activity of the bacteria was greater than that of Gram-negative bacte
5、ria and yeasts; each alkaloid was resistant to the resistant Staphylococcus aureus (MR)The antibacterial ability of Staphylococcus aureus was basically the same. Conclusion the 4 alkaloids of Coptis chinensis are antibacterial constituents of Coptis chinensis franch,The antibacterial spectrum was th
6、e same, but the antibacterial activity was different; the resistance of 4 kinds of Coptis alkaloids to the drug-resistant staphylococcus aureusIt has elimination effect.keyword Coptis chinensis berberine, palmatine, berberine, bacteriostasisAbstract:ObjectiveBerberine, jatrorrhizine, palmatine, andC
7、optisine are four leading alkaloids from Coptidis rhizome. ToResearch the characteristics of antimicrobial activity of aboveAlkaloids, the antimicrobial spectrums and abilities of them wereDetermined in this paper. MethodsSensitivities of 19 microorganisms toBerberine, jatrorrhizine, palmatine, and
8、coptisine were studied underMedicine concentration of 2 000|J g/ml by antimicrobial circleExperiment and their Minimum Inhibitory Concentrations (MIC) against14 sensitive microorganisms were determined by colorimetry in thisStudy. ResultsFour alkaloids from Coptidis rhizome had the sameAntimicrobial
9、 spectrums and different antimicrobial abilities, theAntimicrobial activity of berberine was the strongest, coptisine andPalmatine was relatively weak and jatrorrhizine was the worst. TheAntimicrobial abilities of four alkaloids against G+ bacterium wereStronger than against G - bacterium and fungus
10、. The same activity ofFour alkaloids against Methicitlin resistant Staphylococcus aureus(MRSA) and Staphylococcus aureus were observed.ConclusionAllAlkaloids tested are antimicrobial activity ingredient of CoptidisRhizome. Four alkaloids almost have the same antimicrobial spectumsAnd have different
11、antimicrobial abilities. All alkaloids tested canDispose the drug resistance of Methicitlin resistant StaphylococcusAureus (MRSA)?Key wordsCoptidisRhizome; Berberine; Jatrorrhizine; Palmatine; Coptisine; AnTimicrobial activityCoptis is common in clinical Chinese medicine antibacterial anti-inflammat
12、ory medicine, in addition to the clinical mainly for the treatment of bacillary dysentery,Enteritis, diarrhea, inflammation and other bacterial gastroenteritis disease. Studies have shown that the antibacterial and anti-inflammatory substances in Coptis chinensis FranchThe main basis is the alkaloid
13、 containing berberine,Berberine, Huang Lianjian, andPalmatine is one of the 4 main biological components in Coptis chinensis, and belongs to the isoquinoline in its molecular structureAlkali, with quaternary ammonium group 1. In order to study the antibacterial activity difference and extension of d
14、ifferent alkaloids in Coptis chinensis FranchIn the field of application of Rhizoma Coptidis alkaloids, berberine, berberine, Huang Lianjian and Ba were extracted from the rhizome of Coptis chinensisIn order to test the sensitivity of bacteria to the sensitive bacteria, 19 common plant and animal pa
15、thogens were used as experimental bacteriaThe minimum inhibitory concentration (MIC) was detected in order to extend the use of berberine and other alkaloids in Coptis chinensis FranchProvide scientific basis for clinical medication.1 equipmentExperimental drugs: berberine, berberine, Huang Lianjian and palmatine (extracted from the column of Coptis chinensis by the laboratory)The purity of HPLC was above 97% after separation and preparation. Experimental strain: Staphylococcus aureus(ATCC25923) Staphylococcus aureus resistant bacteria (MR), Mycobacterium tuberculosis, Salmonella t