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1、1742005M8ChemicalResearch andApplication Vo.l17, No. 4Aug. , 2005 l:2004-09-13;:2005-02-17e:(1977-),o,V,1VYk4sT。 Email:yanwuzhusina. comcI|:1004-1656(2005)04-0468-039-(3-&)JT;#s朱燕舞1, 2 ,周运友1(1.=SvS,241000;2.g10HFl, pH5.5-10.0WFviM,VG,Co._(s), 2002, 38(2):101-107. 11,u,., 2002, 10(1):65-67. 12 KumarC
2、 V, AsuncionE H. J. Chem. Soc. Chem.Commun, 1992, 6, 470-475. 13 KumarC V, TurnerR S, AsuncionE H. J. Photochem.Photobiol. A:Chem, 1993, 74, 231-236. 14 Pasternack R F, Brigandi R A, AbramsM J. Inorg.Chem, 1990, 29, 4483-4487.Study and applications of9-(3-am inoanilino)acridinewith DNA fluorescence
3、spectraZHU Yan-wu1, 2, ZHOUYun-you1(1. College ofChemistry andMaterialScience, AnhuiNormalUniversity, Wuhu241000, China;2. SchollofChemical, HefeiUniversity ofTechnology, Hefei230009, China)Abstract:A new fluorescentreagent9-(3-aminoanilino)acridine(m -APAA)wasprepared and the prod-uctwaspurified by
4、 several timesby recrystallition. Its constitutionwasconfirmedby IR, 1HNMR and elementalanalysis. The fluorescence spectra ofm -APAA with Deoxyribonucleic acid(DNA)was studied. Itwas shownthatDNA hasability to quench them -APAA fluorescence in418 nm(ex =256nm), and the quenched inten-sity offluorescence isproportionalto the concentrationDNA. Thismethod had the advantagesofhigh sensitivity,good selectivity and simplicity.Key words:fluorescent reagent;9-(3- aminoanilino)acridine;Determination;DNA(责任编辑李方)470 化学研究与应用第 17卷
